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Diagnostic Antigens of Leishmania.
SEATTLE BIOMEDICAL RESEARCH INST WA
Pagination or Media Count:
We report herein the cloning, expression, and seroreactivity of three Leishmania tropica antigens. By ELISA analysis, the majority of confirmed and suspected viscerotropic leishmaniasis patients had significantly higher levels of specific antibody against two of the three recombinant antigens and the repeat portion of one of the antigens, named Lt-lr, when compared to sera from normal controls. The molecular characterization of these two antigens demonstrated that these are adjacent portions of the same gene and that the repeat portion contains one of the immunodominant epitopes. In addition, this gene was shown to be conserved in all strains of L. tropica parasites recovered from Gulf War participants. The gene does not appear to be highly conserved among all species of Leishmania. Collectively, the data indicate the potential for these antigens to assist in the diagnosis of individuals with viscerotropic leishmamasis.
APPROVED FOR PUBLIC RELEASE