USAF Cellular Mechanism of Turnover of the Stressed Induced Protein HSP70
Final rept. 15 Apr 1993-14 Apr 1994
WYOMING UNIV LARAMIE DEPT OF MOLECULARBIOLOGY
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Because heat shock proteins are made by all organisms in response to environmental stress, it has been proposed that accumulation of these proteins could be useful in environmental monitoring. In order to use the accumulation of heat shock proteins as indicators of environmental stress, it is important to understand how their stability is regulated. This research is concerned with determining the influences that regulate the stability of the major heat shock protein, hsp7O, in rainbow trout used for environmental monitoring and in fruit files a well characterized system used for basic research. During the tenure of this grant progress has been made characterizing the rainbow trout heat shock response, cloning and sequencing the rainbow trout heat shock gene, and in generating antibodies specific for fruit fly and rainbow trout hsp7O. The accumulation of hsp7O in juvenile rainbow trout exposed to heavy metals has been assessed in collaboration with the H. Berman Lab. Commercially available antibodies have been used to identify hsp7O breakdown products in flies, trout, chick and mouse, and the sequences the major breakdown fragments of the fly hsp7O generated in vivo have been determined.
- Medicine and Medical Research
- Stress Physiology