Accession Number:

ADA285295

Title:

Three Dimensional Structure Determination of Botulinum Neurotoxin

Descriptive Note:

Annual rept. 1 Aug 1993-31 Jul 1994

Corporate Author:

CALIFORNIA UNIV BERKELEY SPONSORED PROJECTS OFFICE

Personal Author(s):

Report Date:

1994-08-01

Pagination or Media Count:

28.0

Abstract:

To determine the 3-dimensional structure of botulinum neurotoxins and their isolated domains. The following specifications are listed in the contract section C-Statement of Work Crystallization of the 150 kDs holo-botulinum neurotoxins. Serotype A has been crystallized previously and crystallization conditions will be refined as necessary. Serotype B will be crystallized. Determination of the 3-dimensional structure of serotype A. Heavy atom derivative screening is underway. Once 2 or 3 acceptable derivatives are known, data collection and processing of native and derivative data will be completed. The phases of the x-ray diffraction pattern will be phased, electron density maps calculated, and the structure will be determined

Subject Categories:

  • Radiobiology
  • Microbiology
  • Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE