The effects of Three Hydrocarbons on the Histologic Structure of Male Rat Kidneys
Final rept. 1 Jul 1993-30 Jun 1994
ILLINOIS UNIV AT URBANA COLL OF VETERINARY MEDICINE
Pagination or Media Count:
Using a lysosome specific, acid phosphatase stain developed by our research team, F344 and NBR male rats were found to respond to decalin, JP-4 and JP-8 exposure. Hydrocarbon-induced renal tubular lysosomal alterations were more closely related to the length of exposure rather than the strain of experimental animal. The NBR rats extended exposure had significantly enlarged lysosomes that would often be located in the basal aspect of the renal tubular epithelial cell in a manner similar to the characteristic F344 male rat response, whereas, the F344 rats short exposure showed groups of perinuclear lysosomal aggregates in a manner similar to the characteristic NBR male rat response. This effect could not be detected using, HE, LMBBF, and MH stains. This finding is important in regards to the controversy of alpha 2U-globulins association with hyaline droplet nephropathy because 1 the NBR rat demonstrates significant lysosomal alterations following extended hydrocarbon exposure in the presence of negligible concentrations of androgen-dependent alpha 2U-globulin and 2 the F344 rat demonstrates minimal lysosomal alteration following short hydrocarbon exposure in the presence of high concentrations of androgen-dependent alpha 2U- globulin. Immunohistochemical studies of renal tubular epithelial cells from NBR and F344 male rats exposed to decalin. JP-4 and JP-8 revealed that the microtubules of the cytoskeleton form a characteristic aggregate pattern in the apical portion of the cell in association with hydrocarbon-induced lysosomal alterations. The nephrotoxic effect of decalin, JP-4 and JP-8 appeared to be equivalent as judged by renal tubular lysosomal and cytoskeletal alterations.
- Medicine and Medical Research
- Organic Chemistry