Accession Number:

ADA282921

Title:

Isolation and Preliminary Characterization of a Recombinant TAT Protein from Human Immunodeficiency Virus

Descriptive Note:

Annual rept. 24 May 1993-23 May 1994

Corporate Author:

MORGAN STATE UNIV BALTIMORE MD

Personal Author(s):

Report Date:

1994-06-28

Pagination or Media Count:

56.0

Abstract:

Human Immunodeficiency virus type-1 HIV-1 Tat protein is a transactivator for viral gene expression. In order to carry out the structural and functional studies of the protein, it is necessary to isolate and purify the protein in large quantity. To produce this large quantity, the fusion protein Rop-Tat was over-expressed. The Rop-Tat is isolated from insoluble aggregates of inclusion bodies. Cyanogen bromide cleavage of Rop-Tat yields numerous protein fractions from, S-Sepharose and Q-Sepharose chromatography. Efforts are under way to determine which fractions are related to Tat. Human Immunodeficiency Virus HIV, Transactivator protein Tat, Isolation, Recombinant DNA

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE