A Sensitive, Specific Immunobioassay for Quantitation of Human Interleukin 6
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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Picogram quantities of human interleukin 6 h-IL-6 were detected by a two-step method. A microtiter plate coated with anti-h IL-6 monoclonal antibody was used to capture the IL-6 present in biological samples. An IL-6- dependent B cell line 7TD1 that proliferates in response to IL-6 was added to the captured IL-6. The lower limit of detection for this immunobioassay with serum or cell culture supernatants was 5 pgml. The specificity of the assay was achieved by the antibody used in the first step. The sensitivity was provided by the IL-6-dependent cell line. The method also allows for the removal of inhibitors, metabolites, antagonists or activating agents used to induce IL-6. This immunobioassay has the advantage over other current methods in that it measures immunoactive as well as biologically active IL-6. Immunobioassay, Human interleukin 6, IL-6.
- Medicine and Medical Research