Cytogenetic Studies of testicular Germ Cell Tumors with Chromosome 12 Specific DNA Probes.
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
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The usefulness of interphase cytogenetics in the study of testicular germ cell tumors TGCT was assessed using fluorescence in situ hybridization FISH and a chromosome 12 specific alpha-satellite probe. Nuclei isolated from paraffin-embedded blocks from 79 patients with stage III TGCT were studied after FISH. The overall number, number of large chromosome 12 and small I12P signals, and total signals were measured in hyperdiploid tumor nuclei. These was no evidence of difference in overall signal number or size between seminomas and nonseminomas or among tumor histologies, nor was there evidence of an association between any of the above signal parameters and patient outcome. A ratio of the number of copies of 12p small signals to the number of copies of chromosome 12 large signals appeared to be predictive for patient outcome. As this ratio increased, survival time decreased.
- Medicine and Medical Research