Accession Number:

ADA281927

Title:

Development of a Rapid Chair-Side Test to Detect Pathogenic Bacteria Associated with Periodontal Disease

Descriptive Note:

Final rept. 1 Jan 1989-30 Dec 1993

Corporate Author:

OREGON HEALTH SCIENCES UNIV PORTLAND

Personal Author(s):

• Riviere, George R.

Report Date:

1994-07-07

Pagination or Media Count:

4.0

Abstract:

The purpose of this contract research was to produce a simple and rapid test that would identify dental plaque bacteria associated with early detection of periodontal disease. Spirochetes were studied because of their strong correlation with destructive, progressive periodontal disease. Monoclonal antibodies against Treponema pallidum led to the discovery of a previously unrecognized oral spirochetes in plaque at diseased sites and within gingiva near periodontal lesions. in vitro experiments demonstrated that these pathogen- related oral spirochetes PROS were capable of moving through living tissue no other plaque bacteria and no cultivable oral spirochete were invasive. PROS has been found at sites of periodontal disease in HIV-negative people and in people with HIV-associated periodontal disease. Extensive efforts were made to isolate and cultivate PROS so that PROS-specific monoclonal antibodies and DNA probes could be developed for the chair-side test.

Descriptors:

• *DENSITY
• *BACTERIA
• *HUMAN IMMUNODEFICIENCY VIRUSES
• *DENTAL PLAQUE
• *ANTIBODIES
• TEST AND EVALUATION
• PROBES
• DISEASES
• IN VITRO ANALYSIS
• LESIONS
• SPIROCHAETA
• TREPONEMA PALLIDUM
• TREPONEMA
• CHAIRS
• GRAM NEGATIVE BACTERIA
• MONOCLONAL ANTIBODIES
• CORRELATION
• DIAGNOSIS(MEDICINE)
• DEOXYRIBONUCLEIC ACIDS
• SITES
• DETECTION
• CONTRACTS

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE