Functional Reconstitution of Olfactory Receptor
Final rept. 11 May 1989-3 May 1994
AUBURN UNIV AL
Pagination or Media Count:
We use olfactory receptor neurons and membrane fragments form the rat olfactory epithelium incorporated into planar bimolecular lipid membranes and patch bilayers to define the initial electrochemical membrane events in chemoreception by the mammalian olfactory system. We have found and characterized a chemosensitive K channel from rat olfactory epithelium homogenates which can be functionally reconstituted into artificial planar lipid bimolecular membranes. We have demonstrated the cAMP mimicted the effect of odorant. The statistical analysis of our patch-clamp data suggested the multiple mode of cAMP action on the single channel activity a directly, and b via protein kinase system. We demonstrated, that chemosensitivity of functionally reconstituted olfactory receptor is manifested as a change in the mean open time of single channel events in response to small subnanomolar concentrations of the odorants in the medium bathing the membrane under control of the activity of cyclic nucleotide-processing enzymes.
- Anatomy and Physiology