Accession Number:

ADA281636

Title:

Rapid Detection of Mycobacteria in Patients with HIV Infection

Descriptive Note:

Final rept. 15 Mar 1991-14 Mar 1994

Corporate Author:

ARMED FORCES INST OF PATHOLOGY WASHINGTON DC

Personal Author(s):

Report Date:

1994-05-16

Pagination or Media Count:

13.0

Abstract:

The Polymerase Chain Reaction PCR, along with hybridization to chemiluminescent DNA probes, was used to detect Mycobacteria potentially present in patient specimens from the Mycobacteriology Laboratory at Walter Reed Army Medical Center WRAMC. DNA from specimens were prepared by two different methods, and used in the PCR amplifications. Primers were evaluated using both known and unknown blinded samples. With known samples, the genus specific primers detected 74 of Mycobacteria positive samples, while the M. tuberculosis primers were able to amplify M. Tuberculosis DNA in 59 of the positive samples. With the blinded samples, the genus specific primers were able to detect Mycobacterial sequences in 3 of 8 37.5 of the Mycobacteria-containing samples. There no M. tuberculosis containing specimens in the blinded samples, and all samples were negative for M. tuberculosis by PCR

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE