Effect of Emetine on the Specific Association of T-2 Toxin with Mammalian Cells
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The effects of emetine on the association of T-2 toxin with Chinese hamster ovary cells were examined. T-2 toxin-cell association at both 4 deg C and 37 deg C was reduced by up to 90 after preincubation of cells with emetine. Emetine-induced reduction in T-2 toxin-cell association was time-, temperature-, and concentration-dependent. A 4-min preincubation with emetine at physiological temperature was required to develop the maximum inhibitory effect. After brief exposures or 5 min, emetines inhibitory effects on toxin-cell association were reversible. However, after longer exposure periods to emetine 60 min, toxin-cell association was irreversiby blocked.
- Medicine and Medical Research