DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click HERE
to register or log in.
Effect of Emetine on the Specific Association of T-2 Toxin with Mammalian Cells
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
The effects of emetine on the association of T-2 toxin with Chinese hamster ovary cells were examined. T-2 toxin-cell association at both 4 deg C and 37 deg C was reduced by up to 90 after preincubation of cells with emetine. Emetine-induced reduction in T-2 toxin-cell association was time-, temperature-, and concentration-dependent. A 4-min preincubation with emetine at physiological temperature was required to develop the maximum inhibitory effect. After brief exposures or 5 min, emetines inhibitory effects on toxin-cell association were reversible. However, after longer exposure periods to emetine 60 min, toxin-cell association was irreversiby blocked.
APPROVED FOR PUBLIC RELEASE