Accession Number:

ADA281341

Title:

Effect of Emetine on the Specific Association of T-2 Toxin with Mammalian Cells

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

• Leatherman, Dennis L.
• Middlebrook, John L.

Report Date:

1993-01-01

Pagination or Media Count:

9.0

Abstract:

The effects of emetine on the association of T-2 toxin with Chinese hamster ovary cells were examined. T-2 toxin-cell association at both 4 deg C and 37 deg C was reduced by up to 90 after preincubation of cells with emetine. Emetine-induced reduction in T-2 toxin-cell association was time-, temperature-, and concentration-dependent. A 4-min preincubation with emetine at physiological temperature was required to develop the maximum inhibitory effect. After brief exposures or 5 min, emetines inhibitory effects on toxin-cell association were reversible. However, after longer exposure periods to emetine 60 min, toxin-cell association was irreversiby blocked.

Descriptors:

• *CELLS
• *ISOQUINOLINE ALKALOIDS
• *OVARIES
• REPRINTS
• TEMPERATURE
• TOXICITY
• SYNTHESIS(CHEMISTRY)
• REDUCTION
• REVERSIBLE
• DISSOCIATION
• RIBOSOMES
• ADDITION
• HAMSTERS
• INHIBITION
• TIME
• RATES
• SITES
• PEPTIDES
• ORGANIZATIONS

Subject Categories:

• Medicine and Medical Research
• Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE