Structure-Activity Relationships of Chlorinated Alicyclic Compounds in Catfish
Annual rept. 15 Aug 1992-14 Aug 1993
MISSISSIPPI STATE UNIV MISSISSIPPI STATE COLL OF VETERINARY MEDICINE
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The goal of the EPSCoR project is to conduct these assays in channel catfish Ictalurus punctatus preparations and to make the study a comparative toxicology study. During the first year of the project, Mr Carr has optimized the catfish brain membrane preparation for the assay of 35S-TBPS blinding. He has expanded his background in neurochemistry to learn receptor binding methods and calculation of receptor saturation experiments for the calculation of Kd and Bmax, and he has extended his experience with radioisotope procedures and calculations. He has studied the competition of 12 of the available organochlorine compounds with 35TPBS binding to catfish brain membranes. The compounds selected for study were chosen based on high, moderate and low potency in the rat brain system. The results thus far indicate a much lower concentration of GABA receptors per unit wet weight in catfish brain compared to rat brain. In general, the organochlorine compounds tested thus far have demonstrated similar relative potency in the catfish brain as in the rat brain. Results were submitted for presentation at the Society of Toxicology annual meeting, Dallas, Texas, March, 1994. Studies of the interference of the compounds with 36C1- flux will be initiated in the second year.