PCR (Polymerase Chain Reaction) Testing for Leishmaniasis
SRA TECHNOLOGIES INC ROCKVILLE MD
Pagination or Media Count:
The disease Leishmaniasis, endemic in Africa, South West Asia, and South America, is caused by transmission of a parasite of the Leishmania species via the bite of an infected sandfly. The severity of the disease ranges from cutaneous lesions to a frequently fatal visceralization of the internal organs unless treated at an early stage. Detection of the parasiteis made difficult due to very low numbers of infected cells in peripheral blood. Existing tests for Leishmania parasite are time consuming and have high 50 false negative rates. We report here the development of a rapid polymerase chain reaction PCR based diagnostic capable of detecting 1 infected cell in more than 5 mls of peripheral blood. The test has an accuracy greater than 92, and a false negative rate of less than 8 when validated against known clinical samples. Leishmania, Polymerase chain reaction, Test, RAD I.
- Medicine and Medical Research