Accession Number:

ADA280533

Title:

Chemical and Structural Characterization of Nitroaromatic Adducts with Hemoglobins

Descriptive Note:

Final rept. 15 Jul 1992-30 Sep 1993

Corporate Author:

NATIONAL INST OF STANDARDS AND TECHNOLOGY GAITHERSBURG MD

Personal Author(s):

• Stevens, Walter J.

Report Date:

1994-02-01

Pagination or Media Count:

59.0

Abstract:

The reactivity of dinitrobenzene ortho-, para-, meta-DNB with bovine he has been studied in vitro using spectroscopic and chromatographic methods. Deconvolution of time-dependent VISUV spectra of mixtures of hemoglobin with DNB show that DNB reacts readily with deoxyhemoglobin to form methemoglobin without the need for metabolic activation that has been inferred from previous in vivo studies. No reaction is observed with oxy- or carboxy- hemo globin. Column and HPLC chromatographic separations of hemoglobin treated with 14C radiolabelled DNB did not produce evidence for adduct formation. Similar analyses of hemoglobin from radiolabelled DNB- and TNB-treated rats also showed no direct evidence of strong adducts. Dinitrobenzene, Hemoglobin, Deoxybemoglobin, Carboxyhemoglovin, Nitroaromatic

Descriptors:

• *CLINICAL MEDICINE
• *IN VITRO ANALYSIS
• *HEMOGLOBIN
• ACTIVATION
• TISSUES(BIOLOGY)
• REACTIVITIES
• TIME
• SEPARATION
• METHEMOGLOBIN
• PROTEINS(SIMPLE)
• BOVINES
• CARBON MONOXIDE
• SPECTRA
• MIXTURES
• DEOXYRIBONUCLEIC ACIDS
• RATS
• TOXICITY

Subject Categories:

• Medicine and Medical Research
• Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE