DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
ADA280463
Title:
Ulstrastructural Localization of Tumour Necrosis Factor-Alpha
Descriptive Note:
Corporate Author:
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Report Date:
1994-01-01
Pagination or Media Count:
11.0
Abstract:
The application of an antibody tumour necrosis factor-alpha TNF to thin sections of plastic-embedded mouse tissue has identified sites of TNF activity in normal and endotoxin-treated C3NHeN mice. Prior to endotoxin treatment, TNF was observed in the secretory granules of the antibacterial Paneth cell and one type of crypt endocrine cell. Four hours after endotoxin treatment, these two types of intestinal cell were found to have degranulated. In addition, endotoxin treatment resulted in the appearance of TNF in the secretory granules of all eosinophils, neutrophils and monocytes in the bone marrow, spleen, lung and the proximal intestine. TNF was also observed in the internal elastic lamina IEL of arterioles. These results suggest that the process of TNF induction specifically targets the immune system and the vasculature. An invasive stimulus, such as circulating endotoxin, can provoke the immune cells to be armed with TNF. That same stimulus may cause arteriole smooth muscles cells to secrete TNF. TNF secretion in the presence of arteriole smooth muscle cells may play a role in the adjustment of arteriole tone. In the venules, TaNF may be responsible for platelet and neutrophil accumulation which leads to embolism formation
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
