Accession Number:

ADA280460

Title:

Mast Cell Growth Factor Enhances Multilineage Hematopoietic Recovery in Vivo Following Radiation-Induced Aplasia

Descriptive Note:

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s):

• Patchen, M. L.
• Fischer, R.
• Schmauder-Chock, E. A.
• Williams, D. E.

Report Date:

1994-01-01

Pagination or Media Count:

11.0

Abstract:

Based on in vitro studies, mast cell growth factor MGF also known as steel factor, stem cell factor, and c-kit ligand has been implicated as an important hematopoietic regulator, especially in the presence of additional hematopoietic cytokines. Since hematopoietic regeneration follows sub-lethal radiation-induced hematopoietic injury and is though to be mediated by endogenously produced cytokines, the ability to accelerate recovery from radiation-induced hematopoietic hypoplasia was used to evaluate in vivo effects of MGF administration. Female B6D2F1 mice were exposed to a sublethal 7.75-Gy dose of 60Co radiation followed by subcutaneous administration of either saline or 100, 200, or 400 microgramkgd recombinant murine MGF on days 1 to 17 postirradiation. Recoveries of bone Marrow and splenic spleen colony-forming units CFU-S, granulocyte-macrophage colony-forming cells GM-CFC, and peripheral white blood cells WBC, red blood cells RBC, and platelets PLT were determined on days 14 and 17 during the postirradiation recovery period

Descriptors:

• *MAST CELLS
• *IN VIVO ANALYSIS
• *GROWTH(PHYSIOLOGY)
• REPRINTS
• RADIATION
• WOUNDS AND INJURIES
• HEMATOPOIESIS
• BLOOD CELLS
• MORBIDITY
• COLONIES(BIOLOGY)
• IRRADIATION
• HUMANS
• THERAPY

Subject Categories:

• Biochemistry
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE