Gene Regulation in Memory Formation and Circadian Rhythms
Annual rept. Sep 1992-May 1994
HOUSTON UNIV TX DEPT OF BIOCHEMICAL AND BIOPHYSICAL SCIENCES
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One general objective of this research is to investigate the role of gene expression in circadian rhythms and in memory formation. Another general objective of this research is to develop a new system suitable for both biochemical and molecular studies of circadian rhythms. More specifically, having previously identified a number of proteins that may play important roles in memory formation and circadian rhythms, we wished to explore the function of regulation of expression of the genes for these proteins. Our most important progress to date is in developing techniques suitable for measuring changes in gene expression in the Aplysia nervous system e.g., ribonuclease protection assays and in developing probes for many Aplysia genes calmodulin, BiP, porin, HSP-70, ribosomal mRNA, phosphoglycerate kinease, CEBP, etc.. At this stage, we are at the exciting point where we have just begun to use these techniques and reagents to characterize the regulation of these genes. With regard to the development of model systems for molecular research, we have been unable to observe a circadian rhythm in Halobacteria. We will continue to pursue development of Halobacteria as well as Nematodes and Yeast.
- Anatomy and Physiology