Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases.
Annual rept. 1 Apr 90-31 Mar 91,
YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE
Pagination or Media Count:
Antigens and antibodies were produced and standardized for use in ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for 14 arboviruses and residual infectivity was inactivated with beta- propiolactone. An additional 10 viruses were passaged in mice and the mice were stored frozen awaiting sucrose-acetone extraction of the brains. Rabbits were immunized successfully intravenously with West Nile and Sindbis immunogens grown in RK-13 rabbit cells. ELISA testing of the resulting ammonium sulfate precipitated IgG from West Nile, Sindbis, and other rabbits immunized in past contract periods showed generally excellent titers.
- Medicine and Medical Research