Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases.
Final rept., Annual rept. 1 Apr 87-31 Mar 91,
YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE
Pagination or Media Count:
Reagents were prepared for diagnosis by ELISA of 54 arthropod-borne viruses that cause human disease and are of potential or actual military importance. Mouse brain antigens made by the sucrose-acetone technique to the 54 arboviruses will be useful for assay of arboviral IgG and IgM and may also, in some instances, be used in hemagglutination-inhibition tests. The antigens were inactivated by beta-propiolactone. Rabbit IgG, purified by ammonium sulfate concentration, was prepared to 20 of the arboviruses and was designed to use to capture antigen in ELISA. The titers varied considerably from one rabbit to another, but most may be used in an optimal dilution of 11000 to 132000. Antigens, Antibodies, Arbovirus, Enzyme linked immunosorbent assay
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