Accession Number:

ADA280199

Title:

Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases.

Descriptive Note:

Annual rept. 1 Apr 88-31 Mar 89,

Corporate Author:

YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE

Personal Author(s):

• Shope, Robert E.

Report Date:

1994-05-20

Pagination or Media Count:

6.0

Abstract:

Antigens and antibodies were produced and standardized for use in the ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for Germiston, Qalyub, Sicilian, vesicular stomatitis Indiana, and Ganjam viruses. The antigens were inactivated with beta-propiolactone. Rabbits were immunized successfully intravenously with Ross River, Germiston, and Japanese encephalitis viruses using immunogens grown in RK-13 rabbit kidney cells. These animals were boosted 2 months after the initial immunization. Titers in ELISA for optimal coating of the solid phase ranged between 1500 and 164000. Antigens, Antibodies, Arbovirus, Enzyme linked immunosorbent assay

Descriptors:

• *DIAGNOSIS(MEDICINE)
• *ANTIBODIES
• *ANTIGENS
• *INFECTIOUS DISEASES
• BRAIN
• CELLS
• ENZYMES
• ANIMALS
• ACETONES
• JAPANESE ENCEPHALITIS
• ARBOVIRUSES
• IMMUNOGENS
• INDIANA
• VESICULAR STOMATITIS
• KIDNEYS
• SOLID PHASES
• RABBITS
• IMMUNIZATION
• SUCROSE
• PROPIOLACTONES
• HERPETIC VIRUSES
• JAPANESE ENCEPHALITIS VIRUSES
• ENCEPHALITIS
• VIRUSES
• RIVERS
• EXTRACTION
• SOLIDS
• PHASE
• COATINGS

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE