Role of Line-1 Retrotransposons in Human Breast Cancer.
Rept. for 27 Oct 92-28 Feb 94,
ARMED FORCES INST OF PATHOLOGY WASHINGTON DC
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The human LINE-1 retrotransposon L1Hs is known to cause mutations by inserting into genes and inactivating them. The element is expressed in many breast tumors and breast tumor cell lines, suggesting that L1Hs-induced mutations may play some role in this malignancy. Prior evidence suggested that unmethylated L1Hs elements are transcriptionally active. Therefore, we used the technique of inverse polymerase chain reaction to clone twelve elements that are unmethylated in the breast tumor cell line, T47D. A preliminary characterization has shown that some of these elements are unmethylated in other breast cancer cell lines, but are methylated in a normal breast cell line that does not express L1Hs. These elements are also methylated in several germ cell tumor lines that express L1Hs. Our results to date suggest the possibility that different subsets of active L1Hs elements are expressed in different cancers. LINE-1 Retrotransposon, Breast cancer
- Medicine and Medical Research