Accession Number:

ADA280116

Title:

Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases

Descriptive Note:

Annual rept. 1 Apr 1989-31 Mar 1990

Corporate Author:

YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE

Personal Author(s):

• Shope, Robert E.

Report Date:

1994-05-20

Pagination or Media Count:

7.0

Abstract:

Arbovirus antigens and antibodies were produced and standardized for use in ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for 25 arboviruses and residual infectivity was inactivated with beta-propiolactone. An additional 8 viruses were passaged in mice and the mice were stored frozen awaiting sucrose-acetone extraction of the brains. Rabbits were immunized successfully intravenously with 9 arboviral immunogens grown in RK-13 rabbit cells. Testing of the resulting ammonium sulfate precipitated IgG by ELISA showed generally excellent titers. The testing is continuing.

Descriptors:

• *BRAIN
• *ANTIBODIES
• *ARBOVIRUSES
• *ANTIGENS
• CELLS
• DIAGNOSIS(MEDICINE)
• MICE
• VIRUSES
• PROPIOLACTONES
• SUCROSE
• IMMUNOGENS
• RABBITS
• ACETONES
• INFECTIOUS DISEASES
• SULFATES
• RESIDUALS
• EXTRACTION

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE