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Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases
Annual rept. 1 Apr 1989-31 Mar 1990
YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE
Pagination or Media Count:
Arbovirus antigens and antibodies were produced and standardized for use in ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for 25 arboviruses and residual infectivity was inactivated with beta-propiolactone. An additional 8 viruses were passaged in mice and the mice were stored frozen awaiting sucrose-acetone extraction of the brains. Rabbits were immunized successfully intravenously with 9 arboviral immunogens grown in RK-13 rabbit cells. Testing of the resulting ammonium sulfate precipitated IgG by ELISA showed generally excellent titers. The testing is continuing.
APPROVED FOR PUBLIC RELEASE