Accession Number:

ADA280115

Title:

Effect of Hyperbaric Carbon Dioxide on Spores and Vegetative Cells of Bacillus stearothermophilus

Descriptive Note:

Final rept. 30 Mar-30 Sep 1992

Corporate Author:

ARMY NATICK RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA

Personal Author(s):

Report Date:

1994-05-01

Pagination or Media Count:

38.0

Abstract:

Spore suspensions in water, 0.05M pH 7 phosphate buffer, 0.05M pH 4.5 and 0.05M pH 4.0 acetate buffer, and antibiotic assay media supplement broth AAMS were subjected to 50 psi to 1100 psi carbon dioxide at various temperatures in Parr bombs for up to 96 hours. Spores were resistant to high pressure carbon dioxide treatment at each pH and at both high and low temperatures. Spore suspensions that were first heat activated and then incubated at 550C for a short time increased sensitivity to destruction by CO2. Although spores were resistant to destruction by hyperboric CO2 low pressure 50 psi CO2 was seen to completely inhibit spore germination and outgrowth. Inhibition to germination was found to be reversible. Spore germination was found to be impaired in carbonated AAMS broth. However, the addition of 50 mM naHCO3 to AAMS enhanced germination and outgrowth. Vegetative cells, Bacillus stearothermophilus, Carbon dioxide, Thermophilic bacteria, Spores, Thermophilic Spoilage, Shelf life, Perishable commodities.

Subject Categories:

  • Biology
  • Medicine and Medical Research
  • Inorganic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE