Dengue Type-2 Virus Envelope Protein Made Using Recombinant Baculovirus Protects Mice Against Virus Challenge
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
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The gene coding for the envelope E glycoprotein of dengue 2 virus was cloned into baculovirus Autographa californica nuclear polyhedrosis virus, AcNPV. The recombinant virus contained the entire E protein gene, preceded by 38 nucleotides from the end of the prM gene and followed by the first 83 nucleotides of NS1. When expressed in Spodoptera frugiperda Sf9 cells, about 1 mg of recombinant E antigen was made per 109 cells. This antigen reacted with polyclonal, anti-dengue type 2 antibody and a dengue type 2-specific, neutralizing monoclonal antibody. Balbc mice immunized with the recombinant antigen produced only non-neutralizing antibody against dengue 2 virus but were partially protected against morbidity and mortality after intracranial challenge with virulent dengue 2 virus. Dengue-2 Virus, E Protein, Baculovirus.
- Medicine and Medical Research