Synthesis of Polymeric Microcapsule Arrays and Their Use for Enzyme Immobilization
COLORADO STATE UNIV FORT COLLINS DEPT OF CHEMISTRY
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Immobilized enzymes are used in bioreactors and biosensors. Current methods for immobilizing enzymes include adsorption or covalent attachment to a support, microencapsulation and entrapment within a membranefilm or gel. The ideal enzyme immobilization method would 1 Employ mild chemical conditions 2 Allow for large quantities of enzyme to be immobilized 3 Provide a large surface area for enzymesubstrate contact within a small total volume 4 Minimize barriers to mass transport of substrate and productand 5 Provide a chemically and mechanically robust system. This report describes a new method for enzyme immobilization that satisfies all of these criteria. We have developed a template-based synthetic method that yields hollow polymeric microcapsules of uniform diameter and length. These microcapsules are arranged in a high density array in which the individual capsules protrude from a surface like the bristles of a brush. We have developed procedures for filling these microcapsules with high concentrations of enzymes. The enzyme-loaded microcapsule arrays function as enzymatic bioreactors in both aqueous solution and organic solvents. Enzyme immobilization, Microcapsules, Polypyrrole.
- HIGH DENSITY
- ORGANIC SOLVENTS
- Physical Chemistry
- Polymer Chemistry