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Analysis of Saxitoxin from Urine Using Continuous-Flow Fast Atom Bombardment & Electrospray Ionization Mass Spectrometry
Midterm rept. 1 Apr 1992-31 Mar 1993
MINNESOTA UNIV MINNEAPOLIS
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A method for analysis of saxitoxin STX in urine of rats treated with STX was developed using CBA columns for clean-up of the extract and hydrogen peroxide oxidation prior to analysis by HPLC. Detection of STX is linear between 0.1 and 5 ng when measured by either peak height or area. It was used for analysis of urine from rats treated with 1 microgram total STX. The limit of detection of pure standard is 100 pg. Saxitoxin was found in the urine of the treated rat I pg total dose as soon as 10 min after treatment and up to 8 hrs after dosing. In general, the excretion pattern was bimodal , i e. , the largest amount was found 1 hr after treatment, followed by a decline 2 and 3 hrs afterward, and then a reappearance of larger amounts at 6 and 8 hrs. Adult female rats tolerate a 1 pg dose of STX given by intraperitoneal injection but succumb to 3 to 4 pg i.p. within 3 min.
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