Accession Number:

ADA278874

Title:

Analysis of Saxitoxin from Urine Using Continuous-Flow Fast Atom Bombardment & Electrospray Ionization Mass Spectrometry

Descriptive Note:

Midterm rept. 1 Apr 1992-31 Mar 1993

Corporate Author:

MINNESOTA UNIV MINNEAPOLIS

Personal Author(s):

• Mirocha, Chester J.

Report Date:

1993-04-30

Pagination or Media Count:

29.0

Abstract:

A method for analysis of saxitoxin STX in urine of rats treated with STX was developed using CBA columns for clean-up of the extract and hydrogen peroxide oxidation prior to analysis by HPLC. Detection of STX is linear between 0.1 and 5 ng when measured by either peak height or area. It was used for analysis of urine from rats treated with 1 microgram total STX. The limit of detection of pure standard is 100 pg. Saxitoxin was found in the urine of the treated rat I pg total dose as soon as 10 min after treatment and up to 8 hrs after dosing. In general, the excretion pattern was bimodal , i e. , the largest amount was found 1 hr after treatment, followed by a decline 2 and 3 hrs afterward, and then a reappearance of larger amounts at 6 and 8 hrs. Adult female rats tolerate a 1 pg dose of STX given by intraperitoneal injection but succumb to 3 to 4 pg i.p. within 3 min.

Descriptors:

• *DETECTION
• *MASS SPECTROMETRY
• INJECTION
• RATS
• CHEMISTRY
• OXIDATION
• STANDARDS
• PATTERNS
• ANIMALS
• HEIGHT
• ADULTS
• ANALYTICAL CHEMISTRY
• FEMALES
• METABOLITES
• LABORATORY ANIMALS
• BOMBARDMENT(ATTACK)
• PEROXIDES
• HYDROGEN PEROXIDE
• EXCRETION

Subject Categories:

• Anatomy and Physiology
• Nuclear Physics and Elementary Particle Physics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE