Accession Number:

ADA278720

Title:

Immunoassay of Newcastle Disease Virus and Anti-NDV in Mouse Serum Using a Light-Addressable Potentiometric Sensor

Descriptive Note:

Suffied memo.

Corporate Author:

DEFENCE RESEARCH ESTABLISHMENT SUFFIELDRALSTON (ALBERTA)

Personal Author(s):

• Wikjord, Brenda
• Thompson, H. G.
• Lee, William E.

Report Date:

1994-02-01

Pagination or Media Count:

24.0

Abstract:

A rapid non-radioactive sandwich immunoassay which utilizes biotin- streptavidin mediated filtration capture of immune complexes in conjunction with a silicon sensor was developed for the detection of virus and antibody to virus in serum. Using Newcastle Disease Virus NDV and mouse anti-NDV IgG spiked into mouse serum as a clinical-style model, the lower limits of detection LOD were determined. The LODs per test site were 1 ng for NDV and 4 ng for anti-NDV. The assays were carried out at room temperature in a single step 60 min incubation of immunoreagents with analyte. The assays were easy to perform and required a total time equal to the incubation period plus about ten minutes. The assay format is suitable for a wide range of biological antigens. New assays can be developed and optimized readily, often within one day. The apparatus employs a light-addressable potentiometric LAP sensor is rugged and compact, and requires minimal logistic support. The work presented here demonstrates the utility of the LAP sensor for use in a field or remote hospital setting. Streptavidin, Biotin, Biosensor, Urease, Antibody, Newcastle disease virus, Detection, Serum.

Descriptors:

• *IMMUNOASSAY
• *ANTIGENS
• *NEWCASTLE DISEASE VIRUS
• TEST AND EVALUATION
• TEMPERATURE
• DETECTION
• MODELS
• SITES
• LIGHT
• DISEASES
• ROOM TEMPERATURE
• TIME
• SILICON
• FORMATS
• LOGISTICS
• ANTIBODIES
• INCUBATION
• WORK
• INFECTIOUS DISEASES
• HOSPITALS
• UREASE
• VITAMIN B COMPLEX
• FILTRATION
• BLOOD SERUM
• VIRUSES

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE