Local Immune Response and Protection in the Guinea Pig Keratoconjunctivitis Model Following Immunization with Shigella Vaccines
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
Pagination or Media Count:
This study used he guinea pig keratoconjunctivitis model to examine the importance of route of administration mucosal versus parenteral, frequency and timing of immunization primary versus boosting immunization, and form of antigen givenlive attenuated vaccine strain versus 0-antigen-protein conjugate on the production of protective immunity against Shigella infection. Since local immune response to the lipopolysaccharide LPS 0-antigen of Shigella ssp. is thought to be important for protection against disease, 0-antigen-specific antibody-secreting cells ASC in the spleen and regional lymph nodes of immunized animals were measured using an ELISPOT assay. Results indicated that protective efficacy was associated with a strong 0-antigen-specific ASC response, particularly in the superficial ventral cervical lymph nodes draining the conjunctivae. In naive animals, a strong ASC response in the cervical lymph nodes and protection against challenge were detected only in animals that received a mucosal immunization. Protection in these animals was increased by a boosting mucosal immunization. While parenteral immunization in alone with an 0- antigen-protein conjugate vaccine did not protect naive animals against challenge, a combined parenteral-mucosal regimen elicited enhance protection without the addition of a boosting immunization. Although 0-antigen-specific serum immunoglobin A titers were significantly higher in animals receiving a mucosal immunization there was no apparent correlation between levels of serum antibody and protection against disease.
- Anatomy and Physiology
- Medicine and Medical Research