DNA Polymorphism in the Genome: A Raman Spectroscopic Study
Final rept. 15 Jan-31 Dec 1993
REED COLL PORTLAND OR
Pagination or Media Count:
A laser Raman microscope was used to obtain spectra from polytene chromosomes in transcriptionally active salivary gland nuclei of Drosophila melanogaster third-instar larvae. Bands for DNA, nucleosomes, proteins, and nuclear membrane fatty acids were identified in difference spectra generated from 11 subtraction of the cytoplasm spectra from nuclear spectra. The presence of bands corresponding to B-, C- and disordered-DNA secondary structure indicates that DNA polymorphism exists within salivary gland cells of D. melanogaster.