New Medium for the Culture of Rabbit Sperm for Toxicology Testing
Final rept. Nov 1990-Aug 1992
EDGEWOOD RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
Pagination or Media Count:
A simple salts medium with only glucose as the energy source and buffered by Tris.HCl or HEPES has been developed for the culture of rabbit sperm cells. Cells remain motile for 20 hr and develop hyperactivated motility after incubation at 37 OC for 0.5-1 hr in air. The motility characteristics and parameters such as Vc, AALH, and linearity of the hyperactivated and nonhyperactivated cells are not different from those of cells cultured in the commonly used CO2carbonate buffered media such as DM and T6. This minimal medium extends the use of the rabbit sperm cell for in vitro toxicity testing to include volatile compounds and compounds that form insoluble carbonates, sulfates, or phosphates. Also, the system can be used to assess the potential antifertility effects of chemicals by measurement of changes induced in development of hyperactivated motility by the chemicals. Medium, Videotape, Hyperactivated Rabbit, Toxicity, In vitro, Sperm cells.