Accession Number:

ADA277194

Title:

Development and Use of Medaka as a Model for Immunotoxicity

Descriptive Note:

Corporate Author:

ARMY BIOMEDICAL RESEARCH AND DEVELOPMENT LAB FORT DETRICK MD

Report Date:

1994-01-01

Pagination or Media Count:

13.0

Abstract:

Several species of teleosts, including medaka Oryzias latipes, have been studied with respect to neoplastic responses to known carcinogens. Little information, however, is available concerning the responses of the immune system to environmental chemicals in the medaka. We have initiated studies to characterize immune organs and function in medaka, with the goal of using the medaka as a predictive model of immnunotoxicity in vertebrates. Development of a teleost model for immunotoxicity is part of an integrated biological approach for assessment of the hazard resulting from exposure to complex chemical contaminants in the environment. Medaka immune organs essential for leukopoiesiserythropoiesis as well as cell morphology were examined. Cells isolated from the anterior kidney, spleen and whole blood were characterized with respect to nonspecific esterase activity, myeloperoxidase activity and acid phosphatase activity. in vitro phagocytic cell function following in vitro activation was assessed in primary cultures of pronephros adherent cells. Data collected to date characterizing the endogenous bacterial flora of medaka cultured in our laboratory is also presented.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE