Biocatalytic Processing Polymers in Supercritical Fluids
Final rept. 15 Jun 1990-14 Dec 1993
PITTSBURGH UNIV PA
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The performance and behavior of enzymes in non-aqueous environments has been explored under a variety of conditions, including high temperature and pressure. This work is both of fundamental interest, for providing information on enzyme behavior in non-aqueous environments, as well as of industrial importance, in that commercial processes which could benefit from the use of enzymes often operate in organic solvent, or at temperatures and pressures far from ambient. Subtilisin The work was directed at several topics within this general theme, including a biocatalysis in supercritical fluids a study of the effect of smooth variations in solvent properties on enzyme activity, b biocatalysis in microemulsion systems, a study of the effect of microemulsion system variables on enzyme solubilization and activity, c Spectroscopic investigation of subtilisin in organic solvent a study of the conformation of an enzyme in organic solvent as compared to its conformation in aqueous solution.
- Medicine and Medical Research
- Polymer Chemistry