Accession Number:
ADA275899
Title:
Preclinical Investigation of Lyophilized Platelet Preparations
Descriptive Note:
Triannual rept. year 1, no. 1, 1 Sep-31 Dec 1993
Corporate Author:
EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC
Personal Author(s):
Report Date:
1994-02-02
Pagination or Media Count:
6.0
Abstract:
The characterization of rehydrated human and canine platelets is underway. We have begun the characterization of rehydrated platelets using SDS-PAGE and immunoblotting of human and canine platelet preparations. Rehydrated and fresh platelets were lysed using a freeze-thaw protocol or using a detergent such as Triton X-100. We have found that, as expected, the rehydrated platelets are not lysed with the facility of fresh platelets. SDS-PAGE was performed on the platelet lysates and silver staining was used to visualize the proteins. Our results suggest that rehydrated human platelet lysates have a similar but nonidentical protein profile on SDS-PAGE when compared to fresh human platelet lysates. Similar studies are currently underway using canine fresh and rehydrated platelets.
Descriptors:
Subject Categories:
- Anatomy and Physiology
- Medicine and Medical Research