Accession Number:

ADA275899

Title:

Preclinical Investigation of Lyophilized Platelet Preparations

Descriptive Note:

Triannual rept. year 1, no. 1, 1 Sep-31 Dec 1993

Corporate Author:

EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC

Personal Author(s):

Report Date:

1994-02-02

Pagination or Media Count:

6.0

Abstract:

The characterization of rehydrated human and canine platelets is underway. We have begun the characterization of rehydrated platelets using SDS-PAGE and immunoblotting of human and canine platelet preparations. Rehydrated and fresh platelets were lysed using a freeze-thaw protocol or using a detergent such as Triton X-100. We have found that, as expected, the rehydrated platelets are not lysed with the facility of fresh platelets. SDS-PAGE was performed on the platelet lysates and silver staining was used to visualize the proteins. Our results suggest that rehydrated human platelet lysates have a similar but nonidentical protein profile on SDS-PAGE when compared to fresh human platelet lysates. Similar studies are currently underway using canine fresh and rehydrated platelets.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE