Vertical Transmission of West Nile Virus by culex and aedes Species Mosquitoes
NAVAL MEDICAL RESEARCH UNIT NO 3 CAIRO (EGYPT) DEPT OF MEDICAL ZOOLOGY
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Experiments were conducted to determine whether West Nile WN virus was transmitted vertically by colonized strains of Aedes albopictus, Ae. aegypti, and Culex tritaeniorhynchus. Female mosquitoes were infected by intrathoracic inoculation with WN virus, and the F, progeny were tested for virus by the fluorescence antibody technique and the newborn mouse assay. Each of the three mosquito species transmitted Wn virus to F, adults derived from immature forms reared at 26 deg C. The minimal filial infection rate MFIR ranged from 1 124 to 1 138 for Ae. albopictus. from 162 to 1 172 for Ae. aegypti, and from 1325 to 1859 for Cx. tritaeniorhynchus. The MFIR for Cx. tritaeniorhynchus reared at 20 deg C was 1213 for larvae and 1390 for pupae, and 1208 for larvae and 1554 for pupae reared at 26 deg C. These data are the first reported evidence of vertical transmission of WN virus by mosquitoes, and therefore, warrant further studies to determine whether vertical transmission occurs among WN viral-infected mosquitoes in nature. West Nile virusWN, Vertical transmission, Aedes albopictus, Ae. aegypti, Culex tritaeniorhynchus.
- Medicine and Medical Research