Accession Number:

ADA275291

Title:

DNA Lesions in Medaka (o. latipes): Development of a Micro-Method for Tissue Analysis Using Gas Chromatography-Mass Spectrometry

Descriptive Note:

Mid-term rept. 30 Dec 1991-30 Jun 1993

Corporate Author:

PACIFIC NORTHWEST RESEARCH FOUNDATION SEATTLE WA

Personal Author(s):

Report Date:

1993-07-29

Pagination or Media Count:

27.0

Abstract:

We have reached a point where substantial reductions have been made in the amount of tissue required for GC-MS analysis. In the future, we will analyze the tissues from a number of normal and exposed organisms using the micro method to hopefully validate the approach. The work on monoclonal antibodies is proceeding favorably. Shortly, we expect to obtain a sufficiently pure 8-OH-adenosine to initiate the antigen phase of the work. The FT-IR effort, if successful, will allow for a rapid assessment of DNA modifications in relation to carcinogenesis using as little as a few micrograms of DNA. Overall, the project is on schedule. Fish, Molecular biology, Environmental assessment, RA III.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology
  • Physical Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE