DNA Lesions in Medaka (o. latipes): Development of a Micro-Method for Tissue Analysis Using Gas Chromatography-Mass Spectrometry
Mid-term rept. 30 Dec 1991-30 Jun 1993
PACIFIC NORTHWEST RESEARCH FOUNDATION SEATTLE WA
Pagination or Media Count:
We have reached a point where substantial reductions have been made in the amount of tissue required for GC-MS analysis. In the future, we will analyze the tissues from a number of normal and exposed organisms using the micro method to hopefully validate the approach. The work on monoclonal antibodies is proceeding favorably. Shortly, we expect to obtain a sufficiently pure 8-OH-adenosine to initiate the antigen phase of the work. The FT-IR effort, if successful, will allow for a rapid assessment of DNA modifications in relation to carcinogenesis using as little as a few micrograms of DNA. Overall, the project is on schedule. Fish, Molecular biology, Environmental assessment, RA III.
- Medicine and Medical Research
- Physical Chemistry