Accession Number:

ADA275148

Title:

Estrogen and Hydroxysteroid Sulfotransterases in Guinea Pig Adrenal Cortex: Cellular and Subcellular Distributions

Descriptive Note:

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Report Date:

1993-06-01

Pagination or Media Count:

10.0

Abstract:

This report describes for the first time the cellular and subcellular localization of estrogen sulfotransferase EST as well as the subcellular localization of hydroxysteroid sulfotransferase HST in the mammalian adrenal cortex. A 34-kilodalton EST and two HSTs with 3 alpha- and 3 Beta-hydroxysteroid substrate specificities 32 and 33 kilodaltons, respectively were previously purified from guinea pig adrenal cortex and characterized. Western blots were used to establish that two antisera generated against EST were highly specific for EST, whereas three antisera generated against the HSTs were highly specific for the HSTs, but did not distinguish between the 3 alpha- and 3 Beta HSTs. Light and electron microscopic immunoperoxidase labeling with these antisera revealed that the sulfortransferases were expressed only within the ACTH- responsive layers of the guinea pig adrenal cortex, with EST localized to zona fasciculata and zona reticularis cells, and the HSTs confined to the zona reticularis. No labeling was detected in the zona glomerulosa or adrenal medulla. EST was concentrated in cell nuclei sparse labeling was distributed throughout the cytoplasm. HST labeling was intense in smooth endoplasmic reticulum of zona reticularis cells, but was absent from nuclei.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE