Accession Number:

ADA273787

Title:

AFRRI Reports, Third Quarter 1993

Descriptive Note:

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s):

Report Date:

1993-10-01

Pagination or Media Count:

37.0

Abstract:

The adherence of undifferentiated 51 Cr-labeled HL-60 0.5 x 106 HL- 60 cellswell cells was monitored on influenza virus-infected HUVEC monolayers. Whereas only 3.0 or - 1.6 n 36 of HL-60 cells adhered to uninfected HUVEC, adherence was increased to 41.7 or - 2.2 n 6, 79.7 or - 1.2 n 6, 83.9 or - 0.7 n 6, and 84.4 or - 0.5 n 6 on HUVEC infected for 7 h at a MOI of 1,3,6, and 9, respectively. In comparison, HL-60 cell adherence increased to 35 when HUVEC monolayers were stimulated with LPS 0.2- 20 micrograms for 4 h. Increased adherence to infected HUVEC occurred at 5 h postinfection, peaked at 7 h, and was maintained at 24 h postinfection. Active virus and metabolically active endothelial cells were required to mediate the virus-induced adherence. E-selection and ICAM-1 Ag were upregulated 78.3- and 4. 1-fold, respectively, by LPS 0.02-20 micrograms, 4h whereas virus infection 7 h only increased these proteins 2.6- and 1.4-fold with a MOI greater than or equal 16.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE