Accession Number:

ADA273487

Title:

Hydrogen-Rated System for In Vitro Studies at Pressure: Experimental Validation

Descriptive Note:

Technical rept. Nov 1990-Feb 1991

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1993-10-01

Pagination or Media Count:

50.0

Abstract:

A special hyperbaric perfusion apparatus was constructed to study the neurophysiologic effect of high pressure and the pharmacology of various gaseous agents using the isolated nerve terminal synaptosome tissue preparation. This study was performed to validate the performance of this apparatus in comparison with an alternate device constructed of different materials. This was motivated by theoretical considerations and some anecdotal indications that stainless steel, utilized in the fabrication of the hyperbaric device, might possibly be toxic to synaptosomes. Time course of K-stimulated release of 3 H gaba from guinea pig cerebrocortical synaptosomes was the same for the stainless steel hyperbaric superfusion device as for an apparatus made of plastic. As a practical matter, stainless steel construction material does not appear to affect synaptosome function.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE