Accession Number:

ADA273457

Title:

Effects of Sulfur Mustard on Intracellular Calcium and Synthesis of Basement Membrane Zone Proteins in Human Skin

Descriptive Note:

Midtern rept. 1 Jun 1992-30 May 1993

Corporate Author:

MEDICAL BIOLOGICAL LAB RVO-TNO RIJSWIJK (NETHERLANDS)

Personal Author(s):

Report Date:

1993-08-04

Pagination or Media Count:

29.0

Abstract:

This report presents results obtained during the first year of our study on the effects of sulfur mustard on free intracellular Ca2 levels and synthesis of basement membrane zone proteins in human skin. So far, assays for determination of free intracellular Ca2 levels, ADP-ribosylation and synthesis of basement membrane zone proteins have been almost established and some experiments with cultured human epidermal keratinocytes have been performed. The following preliminary results have been obtained. 1 Exposure of cells to 50 - 200 micrometer sulfur mustard caused little or no effect on free intracellular Ca2 levels during the first 7 hr after treatment. 2 Following exposure of HEK cultured on coverslips to 50 or 250 micrometers sulfur mustard, no changes in the staining intensity of six different basement membrane proteins was observed at 24 hr. 3 The pattern of NAD depletion induced by y- irradiation is different from that induced by sulfur mustard. Sulfur mustard, Human skin, Intracellular calcium, ADP-ribosetransferase, NAD, Basement membrane zone proteins

Subject Categories:

  • Medicine and Medical Research
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE