Comparative Morphology of Sulfur Mustard Effects in the Hairless Guinea Pig and a Human Skin Equivalent
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
Pagination or Media Count:
A commercially available human skin equivalent HSE was used as an in vitro organotypic skin model to study temporal morphological effects of sulfur mustard gas HD. Light and electron microscopic analyses of the HD-human skin equivalent model HD-HSE were compared to the HD-hairless guinea pig model HD-HGP. HSE samples were exposed to 10 micro l HD vapor for 8 min and harvested at selected times up to 24 h. Skin sites of HGP were exposed to the same vapor dose or to 2.0 micro l HD for 30 min and collected at 12 and 24 h. In both models, basal cells of the stratum germinativum were selectively affected. The HD-HSE study revealed that basal cell changes began 3 to 6 h following exposure. These early cellular included an acantholysis of some basal cells with widening of intercellular spaces, disruption of desmosomal attachments, nuclear pyknosis, perinuclear blebbing and repositioning of cytoplasmic tonofilaments to a perinuclear position.
- Anatomy and Physiology
- Chemical, Biological and Radiological Warfare