Accession Number:

ADA268902

Title:

Genetic Variation of HIV: Viral Load and Genotypic Diversity in Relation to Viral Pathogenesis and Treatment

Descriptive Note:

Final rept. 22 Jun 1990-21 Jun 1993

Corporate Author:

ALABAMA UNIV IN BIRMINGHAM

Personal Author(s):

Report Date:

1993-07-20

Pagination or Media Count:

113.0

Abstract:

Elucidation of Human Immunodeficiency Virus HIV pathogenesis and development of effective antiviral treatments and vaccines are priorities of the Army Retrovirus Program which require a thorough understanding of viral replication patterns, viral load burden, and the molecular details of viral variation occurring in natural infection. Previously, little information existed concerning HIV-1 and HIV-2 replication and variation in vivo except for inferences drawn from studies of virus replication in cell culture determinations of circulating p24 antigenemia, and genetic analysis of laboratory isolates of HIV. In our studies, we employed novel PCR polymerase chain reaction, recombinant DNA, and virus culture approaches to study viral replication, burden, and variation in HIV infected individuals and in subjects receiving antiviral treatment. Specific aims were to 1 Develop quantitative PCR-based approaches for measuring HIV-1 and HIV-2 DNA and RNA in blood and bone marrow mononuclear cells PBMCBMMC and in plasma.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE