Accession Number:

ADA265837

Title:

Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1993-04-01

Pagination or Media Count:

5.0

Abstract:

The CD45 protein is a transmembrane tyrosine phosphatase that is required for normal cell receptor TCR-mediated signaling. A chimeric complementary DNA encoding the intracellular enzymatically active portion of murine CD45 preceded by a short amino-terminal sequence from p60 c-src was transfected into CD45 minusT cells. Expression of this chimeric protein corrected most of the TCR signaling abnormalities observed in the absence of CD45, including TCR-mediated enhancement of tyrosine kinase activity and Ca 2 flux. Thus, the enzymatically active intracellular portion of CD45 is sufficient to allow TCR transmembrane signaling

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE