Accession Number:

ADA265659

Title:

Pathogenesis of Campylobacter Fetus Infections: Serum Resistance Associated with High-Molecular-Weight Surface Proteins

Descriptive Note:

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER DENVER CO

Report Date:

1987-04-01

Pagination or Media Count:

12.0

Abstract:

Campylobacter fetus subspecies fetus causes both systemic and diarrheal illnesses. We studied 38 strains of C. fetus isolated from 34 patients underlying illness was present in eight 89 of nine patients with only systemic isolates compared with three 20 of 15 patients with only fecal isolates P .002. In a standardized assay of susceptibility to normal human serum, 27 71 strains were resistant, six 16 had intermediate susceptibility, and five 13 were serum sensitive. Major protein bands migrating a 100 kDa or 125 kDa on polyacrylamide gels were present in all of the 25 serum-resistant strains tested but in only four of seven serum-sensitive isolates of C fetus from humans and animals P .007. The presence of these bands was associated with type A lipopolysaccharide. A low-passaged strain, 82- 40, was serum resistant and contained the 100-kDa protein however, a spontaneous mutant of this strain lacked this band and was serum sensitive. The 100-kDa and 125-kDa proteins of three strains of C fetus were antigenically cross reactive or identical and were exposed on the surface of the C fetus cell. Serum resistance is inherent to most C. fetus isolates from humans and is associated with the presence of cross-reactive surface proteins

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE