Accession Number:

ADA265640

Title:

IL-1 and Tumor Necrosis Factor-Alpha Each Up-Regulate Both the Expression of IFN-Gamma Receptors and Enhance IFN-Gamma-Induced HLA-DR expression on Human Monocytes and a Human Monocytic Cell Line (THP-1),

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1993-02-01

Pagination or Media Count:

7.0

Abstract:

Stimulation of human blood monocytes adherent mononuclear cells and the monocytic cell line, THP-1, by IL-1 or TNF-alpha leads to the up-regulation of IFN-gamma receptors. Scatchard analysis using 125I-IFN-gamma revealed a twofold increase in the number of IFN-gamma receptors on THP-1 cells without an alteration in the affinity of the receptor. The potential functional significance of this induction of IFN-gamma receptors on monocytes and THP-1 cells was investigated by examining the effect of IFN-gamma on MHC class 11 Ag expression by these cells. Both IL-1 and TNF-alpha enhanced the IFN-gamma- induced HLA-DR expression twofold an this effect was inhibited by antibody to IFN-gamma. In the case of human monocytes, IL-1 or TNF-A, each by themselves also increased HLA-DR expression, which was also abrogated by antibody to IFN- gamma. The data suggest that the immunopotentiating effects of IL-1 and TNF- alpha are mediated in part by enhancing IFN-gamma receptor expression on monocytes and macrophages. This presumably would increase the capacity of IFN- gamma to activate macrophages, enabling them to express HLA-DR and present Ag more effectively. Journal of Immunology, 1993, 150 1205

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE