Accession Number:

ADA265567

Title:

Rapid Detection of Mycobacteria in Patients with HIV Infection

Descriptive Note:

Annual rept. 15 Mar 1992-14 Mar 1993

Corporate Author:

ARMED FORCES INST OF PATHOLOGY WASHINGTON DC

Personal Author(s):

Report Date:

1993-05-10

Pagination or Media Count:

12.0

Abstract:

The Polymerase Chain Reaction PCR along with hybridization to chemiluminescent DNA probes was used to detect mycobacteria potentially present in patient specimens from the Mycobacteriology laboratory at Walter Reed Army Medical CenterWRAMC. DNA from the specimens were prepared by two different methods, and used in the PCR amplifications. of the specimens tested 88 were known to contain Mycobacteria. Of these, we were able to detect 20 54, though we were able to detec 82 9 of 11 using a new genus specific primer set. With our M. tuberculosis primers, we were able to detect 21 of 28 samples that were positive by culture results 75. We are continuing to evaluate these primer sets, as well as trying to design a primer set for M. avium.... RA 1, Mycobacteria, Mycobacterium avium, Mycobacterium tuberculosis, Polymerase Chain Reaction, DNA Probes, Rapid identification.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE