Safety and Immunogenicity of a Live-Attenuated Junin (Argentine Hemorrhagic Fever) Vaccine in Rhesus Macaques
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The safety and immunogenicity of Candid 1, a live-attenuated Junin virus vaccine, were elevated in rhesus macaques. Candid 1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units PFU into four groups of five animals each four controls received saline. There was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. Junin virus was recovered by cocultivation from peripheral blood mononuclear cells PBMC of 14 70 of 20 animals from 1 to 21 days after immunization 27 12 of 223 PBMC samples that represented animals in all four dose groups were positive. In contrast, virus was recovered from the plasma of only two of 20 macaques two of 225 samples 0. 9, and only once by amplification from throat swabs. No evidence of reversion was detected in any blood isolate. All animals developed a detectable neutralizing antibody response following vaccination. These results indicate that Candid 1 is safe and immunogenic in nonhuman primates.
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