Accession Number:

ADA265461

Title:

Two-Dimensional Gel Electrophoresis and Immunoblotting of Campylobacter Outer Membrane Proteins

Descriptive Note:

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER DENVER CO

Report Date:

1987-04-04

Pagination or Media Count:

10.0

Abstract:

We characterized outer membrane proteins OMPs from selected Campylobacter jejuni. C. coli, and C. fetus strains by two-dimensional gel electrophoresis 2DGE, using isoelectric focusing and sodium dodecyl sulfate- polyacrylamide gel electrophoresis SDS-PAGE, and by immunoblotting with immune rabbit serum. The flagellar band with a molecular mass of 63 kilodaltons kDa demonstrated previously by one-dimensional SDS-PAGE was shown by 2DGE to consist of one or several charge trains, depending upon the species, strain, and type of preparation studied each of the individual peptides was found to be antigenic by immunoblotting. In contrast, in all of the strains studied, the major OMP 43 to 44 kDa of C. jejuni and C. coli consisted of a single isomeric form which was weakly immunogenic. Several minor proteins 29 to 31 kDa were found to be strongly immunogenic by immunoblotting. C. fetus strains possessed two major OMPs of 45 to 47 kDa, each of which consisted of either a single isomer or a major isomer comprising at least 90 of the major OMP. Serum-resistant strains of C. fetus possessed an acid-labile 100-kDa glycoprotein pI. 4.1 which was markedly diminished or absent in serum-sensitive strains. These 2DGE analyses provide information that is useful in taxonomic and epidemiologic studies and for the purification of surface antigens for the development of campylobacter vaccines and may also facilitate the identification of specific virulence factors

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE