Accession Number:

ADA265460

Title:

Antigenicity of Campylobacter Jejuni Flagella

Descriptive Note:

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER DENVER CO

Report Date:

1986-03-28

Pagination or Media Count:

7.0

Abstract:

We studied the antigenicity of a wild-type flagellate and motile F M Campylobacter jejuni strain 81116 and two daughter mutants, one flagellate and immotile F M- and one aflagellate and immotile F-M-. By solium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton kDa band identified from sheared flagella as the flagellar protein was present in the FM and FM- strains but not in the F-M- strain. No other differences in protein profile among the three strains were noted. By Western blotting, serum from rabbits immunized with either the FM or F-M- strain detected a 63-kDa protein in the FM and FM- strains but not in the F-M- strain. That the F-M- antiserum recognized the 63-kDa band suggests that small amounts of this protein or a cross-reacting antigen is present on the F-M- strain. By counterimmunoelectrophoresis of the acid-extracted preparations with immune sera, all three strains were found to share three major antigens, but a fourth antigen with a net positive charge was present only in the FM and FM- strains. Antisera to five C. jejuni and two Campylobacter fetus strains recognized the 63-kDa protein oif purified FM flagella in Western blots, demonstrating that a common antigen is present, but enzyme-linked immunosorbent assay results suggest that the sharing of this antigen among Campylobacter strains is variable

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE