Accession Number:

ADA263240

Title:

Evaluation of Dried Storage of Platelets for Transfusion: Physiologic Integrity and Hemostatic Functionality

Descriptive Note:

Annual rept. no. 1, 1 Feb 1992-31 Jan 1993

Corporate Author:

EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC

Report Date:

1993-01-31

Pagination or Media Count:

9.0

Abstract:

The intramural collaboration at East Carolina University to study metabolic activity of lyophilized platelets was cancelled after six months due to departure of the investigator. This study will now be carried out by colleagues at the American Red Cross Research Laboratory in Norfolk, VA. Samples of various platelet preparations have been sent and analysis is underway. The major objectives achieved at ECU in the first year were initiation of long-term storage studies of the standard lyophilized platelet preparation, set-up of Baumgartner chamber methodology for assessment of platelet adhesiveness, and further development of permanganate-based stabilization protocols before platelet lyophilization. A long-term study of the stability of surface receptors on lyophilized platelets stored under various conditions desiccated, at room temperature or 4 deg C, or frozen at -70 deg C was initiated in June, 1992, with a single large batch of paraformaldehyde-treated dried human platelets. At one month intervals, sample vials from each storage environment were reconstituted and assayed by flow cytometry with monoclonal antibody probes to the major surface glycoproteins GPlb, GPlbIX complex, GPllbllla, and GPIV. The only significant finding thus far 6 months is a decrease in binding of MoAb SZ-1 to the dried platelets stored at room temperature.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE