Further Development and Validation of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX).
Phase 1 Progress rept.,
OKLAHOMA STATE UNIV STILLWATER DEPT OF ZOOLOGY
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The Frog Embryo Teratogenesis Assay Xenopus FETAX is a 96 hour whole embryo developmental toxicity screening assay. It was designed to detect xenobiotics that selectively impair embryo development, growth and survival developmental toxicants at concentrations far less than those required to in- pair adults. Not all xenobiotics act in this manner. Consequently, it is necessary to use tests that include developmental endpoints or risk missing toxic effects on a weak link in the life cycle of vertebrates. The purpose of the contract was to establish the repeatability and reliability of FETAX by conducting an interlaboratory study in three phases. The results of Phase I employing Hydroxyurea. Isoniazid and 6-Aminonicotinamide are presented here. The same lot numbers were used throughout the study so that variation in chemical purity did not affect results. Identity of the compounds was known. Three concentration response tests were performed for each compound in each laboratory. There was good correlation between historical data and new test results especially for isoniazid and 6-aminonicotinamide. Results suggest that Phase II should be Frog embryo teratogenesis assay - xenopus, FETAX, ILS, Validation, Developmental, Toxicology, Teratology, Lab animals, RAD III, Phase I, performed. The contract called for participation in an annual workshop and for technology transfer. The results from the workshop and the book chapter for Fundamentals of aquatic Toxicology are provided.